Michael Douglas, Microbiologist
Kansas Health and Environmental Laboratories
6700 SW Topeka Blvd, Bldg 740
Topeka, KS 66619-1401
Telephone Number: 785-296-1635
The Mycobacteriology section identifies Mycobacterium species, with an emphasis on detection of Mycobacterium tuberculosis. Patient specimens are processed and analyzed by fluorescent microscopy and liquid culture. Smear positive specimens from respiratory sites that are identified by microscopy are further tested by nucleic acid amplification for rapid detection of M. tuberculosis. Liquid culture positive specimens, after confirming the presence of acid-fast bacilli by microscopy, are analyzed by high performance liquid chromatography (HPLC), which can identify Mycobacterium species or groups. Reference culture isolates sent to the laboratory are also confirmed by microscopy and tested by HPLC. The Mycobacteriology laboratory also performs susceptibility testing on all Mycobacterium tuberculosis isolates, with automatic referral to the CDC for any isolate determined resistant to primary drug therapy (streptomycin, isoniazid, rifampin, ethambutol, and pyrazinamide).
Blood samples require special collection procedures, and should be coordinated with the laboratory to increase chances of mycobacteria recovery.