Pulse Field Gel Electrophoresis Laboratory

Kansas Health and Environmental Laboratories
6810 SE Dwight Street
Topeka, KS 66620
Telephone Number: (785) 296-1633
Email: kdhe.microlab@ks.gov

Pulsed-Field Gel Electrophoresis (PFGE) is used to create a DNA fingerprint to track bacterial strains associated with foodborne outbreaks across the states and countries. When E. coli O157:H7, Shiga-toxin producing E. coli (STEC), Shigella, or Salmonella is identified, the organism is fingerprinted by PFGE, and its pattern is compared to other patterns from that same serotype throughout the states and countries. Any fingerprint pattern that matches within a similar time frame are brought to the attention of the Bureau of Epidemiology and Public Health Informatics and the Centers for Disease Control and Prevention (CDC) in Atlanta, GA.

Additional information on PFGE can be found at http://www.cdc.gov/pulsenet/.

Salmonella Fingerprint Patterns

Salmonella fingerprint patterns.
Each vertical lane represents a different Salmonella strain.